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首页> 外文OA文献 >CYTOLOGICAL STUDIES OF ORGANOTYPIC CULTURES OF RAT DORSAL ROOT GANGLIA FOLLOWING X-IRRADIATION IN VITRO : I. Changes in Neurons and Satellite Cells
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CYTOLOGICAL STUDIES OF ORGANOTYPIC CULTURES OF RAT DORSAL ROOT GANGLIA FOLLOWING X-IRRADIATION IN VITRO : I. Changes in Neurons and Satellite Cells

机译：体外X射线辐照大鼠背根神经节组织文化的细胞学研究：I。神经元和卫星细胞的变化

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Long-term organotypic cultures of rat dorsal root ganglia were exposed to a single 40 kR dose of 184 kvp X-rays and studied in the living and fixed states by light or electron microscopy at 1–14 day intervals thereafter. Within the first 4 days following irradiation, over 30% of the neurons display chromatolytic reactions (eccentric nuclei, peripheral dispersal of Nissl substance, central granular zone) as well as abnormal nucleolar changes and dissociation of ribosomes from endoplasmic reticulum cisternae. Some satellite cells undergo retraction or acute degeneration, leaving only basement membrane to cover the neuron in these areas. 8 days after irradiation, neurons also exhibit (a) areas in which ribosomes are substantially reduced, (b) regions of cytoplasmic sequestration, (c) extensive vacuolization of granular endoplasmic reticulum and Golgi complex, and (d) diversely altered mitochondria (including the presence of ribosome-like particles or association with abnormal glycogen and lipid deposits). Nucleolar components become altered or reoriented and may form abnormal projections and ringlike configurations. Sizeable areas of the neuronal soma are now denuded of satellite cells; underlying these areas, nerve processes are found abnormally invaginated into the neuronal cytoplasm. By the 14th day following irradiation, most neurons display marked degenerative changes including extensive regions of ribosome depletion, sequestration, vacuolization, autolysis, and, in some areas, swirls of filaments, myelin figures, and heterogeneous dense bodies. These observations demonstrate that X-irradiation produces profound cytopathological changes in nervous tissue isolated from the host and that many of these changes resemble the effects of radiation on nervous tissue in vivo.
机译：将大鼠背根神经节的长期器官型培养物暴露于40 kR剂量的184 kvp X射线下，然后在1-14天的间隔内通过光镜或电子显微镜在活体和固定状态下进行研究。在照射后的前4天内，超过30％的神经元显示出色谱反应（离心核，尼氏物质的周边扩散，中央颗粒区）以及异常的核仁变化和核糖体从内质网池的解离。一些卫星细胞会发生退缩或急性退化，仅留下基底膜覆盖这些区域的神经元。辐照后第8天，神经元还表现出（a）核糖体基本减少的区域，（b）胞质螯合的区域，（c）颗粒状内质网和高尔基体的广泛空泡化，以及（d）线粒体（包括核糖体样颗粒的存在或与异常糖原和脂质沉积的关联）。核仁成分发生改变或重新定向，并可能形成异常突起和环状构型。现在，神经元躯体的相当大的区域被卫星细胞剥夺了。在这些区域的下面，发现神经过程异常侵入神经细胞质。照射后第14天，大多数神经元显示出明显的退行性变化，包括核糖体耗竭，螯合，空泡化，自溶的广泛区域，在某些区域还出现细丝漩涡，髓磷脂图形和异质致密体。这些观察结果表明，X射线辐照会在与宿主分离的神经组织中产生深远的细胞病理学变化，并且其中许多变化类似于放射线对体内神经组织的影响。

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